Computational protocol: Rapid and Sensitive Isothermal Detection of Nucleic-acid Sequence by Multiple Cross Displacement Amplification

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Protocol publication

[…] Listeria monocytogenes (L. monocytogenes) was chosen as a model target microorganism for demonstrating the availability of MCDA assay. For the L. monocytogenes-specific hlyA gene, a set of 10 primers, which targeted 10 distinct regions, was designed based on the mechanism of MCDA by using PrimerExplorer V4 (Eiken Chemical) and primer software PRIMER PREMIER 5.0. The locations and sequences of MCDA primers were shown in and . […]

Pipeline specifications

Software tools PrimerExplorer, Primer Premier
Application qPCR