Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 22
Release date: Aug 1 2016
Last update date: Aug 20 2018
Access: Public
Diseases: Neoplasms, Warts, Tumor Lysis Syndrome
Dataset link Characterization of active piRNAs and piRNA machinery in WRR-1 cells deficient for the Hippo pathway (deficient for the warts tumor suppressor) and expressing activated RasV12 (RNA-seq)

Experimental Protocol


Small RNAs cloned from whole cells, Piwi-bound small RNAs and long RNA-Seq were performed in wts-RNAi;RasV12 cells to identify piRNAs and gene expression changes compared to RasV12 cells, somatic OSS cells, and germline UAS-wts-RNAi;UAS-RasV12 control ovaries. After knock-down of components of the piRNA machinery in WRR-1 cells ([piwi, zuchini, armitage, aubergine, argonaute3) and GFP and/or dsRED control knock-downs, small RNAs were cloned and gene expression (RNA-Seq) profiles were established.

Repositories


GEO

GSE83843

ArrayExpress

E-GEOD-83843

ENA

SRP077568

BioProject

PRJNA327202

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