Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Feb 25 2013
Last update date: Aug 23 2018
Access: Public
Diseases: Neoplasms, Neuroblastoma, Hematologic Neoplasms
Dataset link Targeting MYCN in Neuroblastoma by BET Bromodomain Inhibition

Experimental Protocol


JQ1 is a novel thieno-triazolo-1,4-diazepine, which displaces BET bromodomains from chromatin by competitively binding to the acetyl lysine recognition pocket. BE(2)-C and Kelly cells were treated in triplicate with 1 ┬ÁM JQ1 or DMSO for 24 hours. RNA was extracted and a decrease in MYCN transcript was confirmed by real time RT-PCR as described above. The samples were profiled using the Affymetrix PrimeView Human Gene Expression Array (Affymetrix) at Beth Israel Deaconess Medical Center (Boston, MA, USA).

Repositories


GEO

GSE43392

ArrayExpress

E-GEOD-43392

BioProject

PRJNA185813

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Contact


Gabriela Alexe