|Application:||aCGH data analysis|
|Number of samples:||4|
|Release date:||Sep 18 2015|
|Last update date:||May 4 2016|
|Diseases:||Brain Neoplasms, Carcinoma, Renal Cell, Kidney Neoplasms|
|Dataset link||Single-cell transcriptome profiling for metastatic renal cell carcinoma patient-derived cells [aCGH]|
In order to identify successful clonal propagation from patient to PDX samples and understand pathogenesis from primary to metastatic RCC, we performed whole-exome sequencing (WES, n=4) and matched aCGH (n=4) on bulk tumor samples. And we utilized single-cell RNA sequencing (scRNA-seq) to model and dissect functional heterogeneity acroass primary and metastatic RCC tumors. We checked whether of capturing live one cell, not more cells, in microfluidics by fluorescent microscopic observation. To construct RNA sequencing libraries, we performed further quality controls including adequate quantities and qualities of amplified transcriptomes respectively from single cells. Tumor cells from the parental mRCC (n=34), PDX-mRCC (n=36) and PDX-pRCC (n=46) were finally analyzed in this study after filtering out poor quality cells.
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