Computational protocol: Molecular Characterization of Taenia multiceps Isolates from Gansu Province, China by Sequencing of Mitochondrial Cytochrome C Oxidase Subunit 1

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Protocol publication

[…] All of the amplicons were ligated into pMD-18 T vector (Takara) and transformed to Escherichia coli DH5α competent cells. The positive colonies were sequenced with ABI PRISM 3730 Genetic Analyzer, and sequences were analyzed with DNAtools software. Further comparison with taeniid cox1 gene sequences available in GenBank was made using the BLAST and Clustal W software. Phylogenetic analyses were performed using the neighbor joining (NJ), maximum parsimony (MP), and minimum evolution (ME) methods, and the Mega 5.05 program. The bootstrap value was set as 1,000 replications with a cutoff value of 50%. […]

Pipeline specifications

Software tools Clustal W, MEGA
Application Phylogenetics
Organisms Ovis aries, Capra hircus, Taenia multiceps
Diseases Taeniasis