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[…] repared, following and electron micrographs were captured with a FEI Tecnai SPIRIT transmission electron microscope (FEI, Eidhoven, Netherlands), operated at 120 kV and equipped with an EAGLE CCD Camera., Total RNA was isolated with miRNeasy micro kit (Qiagen), assessed with Nano Drop ND-1000 Spectrophotometer (Peqlab) and reverse transcribed to cDNA. qPCR, performed with SYBR Green (Thermo Fisher Scientific or Qiagen). miRNA/mRNA levels were normalized to U6/hypoxanthine phosphoribosyltransferase 1 (Hprt), respectively. Primer sequences are described in Table . Library prep for NGS, following [46] and sequencing performed on Illumina 2500 at 50 bp single read. Fasta files created (Illumina CASAVA-1.8.2 software) and mapped (TopHat2 version v2.0.10) against the mouse genome, build mm9. Approximately, 85–90% mapping rate was observed. Only uniquely mapped reads determined the number of reads per gene (HTSeq-count script 0.6.1p1). Differentially expressed genes, were determined by padj < 0.05 and an absolute fold change >2 (DESeq. 2 package v1.4.5) and hierarchical clustering using Pearson dissimilarity and complete linkage was performed to explore gene expression patterns (Matlab 8.0.0.783)., Analysis was performed manually or with GraphPad Prism 6 for Student’s t-test, test of proportion or one way ANOVA with post-hoc Newman-Keuls or one way ANOVA with Duncan’s new multiple range test (MRT), as indicated. Results are given as mean ± standard error of the mean (s.e.m). The null hypothesis was rejected at the 0.05 level (*), 0.01 (**) or 0.001 (***). Non-significant values on statistical test are not mentioned in the figures. Gene Ontology analysis was performed using DAVID., , Tal Yardeni and Raquel Fine contributed equally to this work., Electronic supplementary material , Supplementary information accompanies this paper at 10.1038/s41598-017-17878-x., Publisher's note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations., We thank Jing Liang and Ayelet Avin for technical support, Oded Singer for viral prep, Smadar Levin Zaidman for electron microscopy, Atan Gross, Ido Amit and Eli Arama for reagents and advice (Weizmann Institute of Science); Adi Laser-Azogui and Roy Beck (Tel Aviv University) for generously sharing Vimentin overexpression lentivirus. Heather D. Durham, (McGill Univ […]

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