Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 7
Release date: Nov 10 2012
Last update date: May 11 2017
Access: Public
Diseases: Diabetes Mellitus, Type 2, Glucose Intolerance, Glucose Metabolism Disorders
Chemicals: Glucose
Dataset link Adenoviral shRNA-based knockdown of hepatic Hnf1b (Ad-shHnf1b)

Experimental Protocol


Adenoviruses (Ad5) encoding either GFP (Ad-Ctrl 1-3) or shHnf1b (Ad-shHnf1b 1-4) were injected into the tail vein of C57BL/6 mice at 1x10E10 viral particles (VP) per gram bodyweight. Biotin-labeled cDNA was synthesized using GeneChip Whole Transcript Sense Labeling Assay (Affymetrix) according to vendor’s instructions. After fragmentation, cDNAs were hybridized for 17h at 45°C on Affymetrix Mouse Gene 1.0 ST Arrays. The Arrays were washed and stained in the GeneChip Fluidics Station 450 and scanned on a GeneChip Scanner 3000 7G (Affymetrix). Data intensities were log transformed and normalized with a quantile normalization method using Affymetrix Power Tools. Differentially expressed genes were identified according to statistical evidence indicated by Student's t-test and fold change statistics

Repositories


GEO

GSE42188

ArrayExpress

E-GEOD-42188

BioProject

PRJNA179265

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Contact


Jan-Wilhelm Kornfeld