Computational protocol: Clock-controlled mir-142-3p can target its activator, Bmal1

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Protocol publication

[…] Three miRNA target prediction databases (TargetScan, PicTar and MicroCosm) were used to predict Bmal1/Clock-targeting miRNAs. The potential targets of mir-142-3p and -5p were predicted using the TargetScan and PicTar algorithms, and the targets predicted by both algorithms were listed in Additional file . The conserved E-box (CACGTG) in the upstream regulatory sequence of mir-142 was analyzed using DNAman, and the conservation track was obtained from the UCSC genome browser ( http://genome.ucsc.edu/). […]

Pipeline specifications

Software tools TargetScan, PicTar, DNAMAN
Databases UCSC Genome Browser
Application Genome data visualization
Organisms Mus musculus, Homo sapiens