Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 56
Release date: Aug 23 2010
Last update date: Jan 18 2013
Access: Public
Diseases: Respiratory Distress Syndrome, Adult, Acute Lung Injury
Chemicals: Oleic Acid
Dataset link Gene Expression Profiles Characterize Inflammation Stages in the Acute Respiratory Distress Syndrome in Mice

Experimental Protocol


Wild-type female C57Bl/6J mice, 7 weeks old, were obtained from Charles River and housed in a specific pathogen-free animal facility. We studied 36 mice divided into 6 groups. Each group included one or two mice administered with physiological serum and 3 to 7 mice administered with OA. Each group can be distinguished given the incubation time: 1H, 1H30, 3H, 4H, 18H and 24H. All experimental procedures involving animals were approved by the veterinary office of the Ministry of Agriculture, France (authorization number: 13-27). 16 mRNA samples extracted from an individual organ were run on a single microarray. In addition, 22 samples were run on two microarrays, and were considered as technical replicates. This corresponds to a total of 60 microarrays. 9 microarrays were hybridized with mice administered with physiological serum and 51 were hybridized with mice administered with oleic acid.

Repositories


GEO

GSE18712

BioProject

PRJNA121509

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Contact


Isabelle Lesur Kupin

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