Computational protocol: The amino terminal F-box domain of Petunia inflata S-locus F-box protein is involved in the S-RNase-based self-incompatibility mechanism

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Protocol publication

[…] All DNA sequencing was carried out at the Nucleic Acid Facility of The Pennsylvania State University (http://tanager.huck.psu.edu). Nucleotide sequences were assembled and analysed using DNA Strider 1.2.1. Alignments of amino acid sequences were performed using ClustalW (http://www.ebi.ac.uk/clustalw/); gonnet250 protein weight matrix was selected, and the gap opening and extension parameters were 10 and 0.2, respectively. Alignments were shaded using Boxshade version 3.21 (http://www.ch.embnet.org/software/BOX_form.html). […]

Pipeline specifications

Software tools Clustal W, BoxShade
Application Proteome data visualization
Organisms Arabidopsis thaliana
Diseases Protein S Deficiency