Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 116
Release date: Aug 20 2013
Last update date: Nov 19 2013
Access: Public
Chemicals: Amino Acids
Computational protocol: Comet, DTASelect
Dataset link Effects of Diet on Resource Utilization by a Model Human Gut Microbiota Containing Bacteroides cellulosilyticus WH2, a Symbiont with an Extensive Glycobiome (RNA-Seq)

Experimental Protocol


116 samples total. In 26 of these samples, we evaluated community-wide gene expression using RNA isolated from the feces of a gnotobiotic mouse harboring an artificial community comprised of 12 human gut microbes. For these samples, we sought to determine the extent to which community gene expression is altered as a result of dietary oscillation. In the other 90 samples, we evaluated gene expression in a single species (B. cellulosilyticus WH2) grown in a defined medium supplemented with a single mono-, oligo-, or polysaccharide. For these samples, we sought to identify genes (particularly polysaccharide utilization loci) whose expression was significantly increased as a result of exposure to particular carbohydrates.

Repositories


GEO

GSE48993

ArrayExpress

E-GEOD-48993

ENA

SRP027547

BioProject

PRJNA212536

Download


Contact


Nathan McNulty
Nathan P McNulty

Dataset Statistics

info

Citations per year

Dataset publication