Computational protocol: Platycarya strobilacea S. et Z. Extract Has a High Antioxidant Capacity and Exhibits Hair Growth-promoting Effects in Male C57BL/6 Mice

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Protocol publication

[…] To determine the levels of cytokines involved in hair growth, the mRNA expression of 5 cytokines, insulin-like growth factor (IGF)-1, keratinocyte growth factor (KGF), and transforming growth factor (TGF)-β1 were relatively quantified. cDNA was synthesized by reverse transcription after total mRNA extracted from the dorsal skin tissue of each C57BL/6 mouse. Polymerase chain reaction (PCR) was used to amplify each gene of interest after mixing the resulting cDNA with each gene primer set.IGF-1 and KGF were quantified by gene amplification (denaturation, 30 s at 95ºC; annealing, 60 s at 60ºC; and extension, 30 s at 72ºC) by PTC-150 MiniCycler (MJ Research, Inc., Waltham, MA, USA), dielectrolysis (Bio-Rad, Hercules, CA, USA), image capturing (Gel Doc XR UV transilluminator; Bio-Rad), and digitizing band length (MBF ImageJ, Version 6.0, MBF Bioscience Inc., Williston, VT, USA). TGF-β1 was quantified using real-time PCR (TCR0096 Thermo Fisher Scientific Inc.), Waltham, MA, USA) for gene amplification (denaturation, 15 s at 95ºC; annealing, 15 s at 60ºC; and extension, 40 s at 72ºC) and VeriQuest SYBR Green qPCR Master Mix (2X) (Affymetrix Inc., Santa Clara, CA, USA). The primer sets were purchased from Bioneer and used at a 100 pmol concentration in DEPC-DW; the primer sequence of each gene is shown in . [...] Collected data were analyzed by one-way ANOVA using the SPSS Statistics vers 17.0 (SPSS Inc., Chicago, IL, USA). Duncan’s multiple range test was used for post-hoc analysis. Differences between groups were considered significant when P<0.05. All experiments were repeated at least 3 times to assess reproducibility, and data are provided as mean±standard error (SE). […]

Pipeline specifications

Software tools ImageJ, SPSS
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Mus musculus
Diseases Drug-Related Side Effects and Adverse Reactions
Chemicals Dimethyl Sulfoxide, Eosine Yellowish-(YS), Hematoxylin, Minoxidil