Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Access: Public
Taxon: Escherichia coli, Pseudomonas aeruginosa, Caenorhabditis elegans
Computational protocol: Trim Galore!, cutadapt, BWA, GATK, SnpEff, Picard, SAMtools, SRA
Dataset link Whole-genome microarray of Caenorhabditis elegans strains DA650 and RC301 exposed to Pseudomonas aeruginosa PA14

Experimental Protocol


DA650 and RC301 animals were synchronized to developmental stage L1. These animals were grown at 20°C on nematode growth media plates seeded with Escherichia coli OP50 until L4 stage (48 hours). At 48 hours, animals were transferred to plates containing Pseudomonas aeruginosa strain PA14. After 4 hours of incubation at 25°C, animals were rinsed off the plates and total RNA from three biological replicates was extracted. C. elegans Gene Expression Microarray (Agilent Technologies, Santa Clara, CA) were used.were used.

Repositories


GEO

GSE86431

BioProject

PRJNA341857

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Contact


Alejandro Aballay

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