Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 7
Release date: Jul 18 2016
Last update date: Jan 23 2018
Access: Public
Diseases: Breast Neoplasms, Neoplasms, Prostatic Neoplasms
Chemicals: Androgens, Estrogens
Dataset link Chromatin targets of androgen receptor and PIAS1 in molecular apocrine breast cancer cells [ChIP-seq]

Experimental Protocol


All ChIP-seq samples were collected from cells treated with 10 nM R1881 (synthetic androgen methyltrienolone) for 2 h. In addition, AR ChIP-seq samples were collected from cells exposed to control siRNA (siNON, Dharmacon Non-targeting pool) or PIAS1 siRNA (siPIAS1, Dharmacon ON-TARGETplus SMARTpool for human PIAS1). IgG sample was collected from siNON and siPIAS1 cells and used as background control. Biological duplicate samples of the AR, PIAS1 and single IgG ChIP-seq samples were analyzed by using Solexa/Illumina 1.5.

Repositories


GEO

GSE70161

ENA

SRP059781

BioProject

PRJNA287765

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Contact


Sari Toropainen