Dataset features


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Feb 3 2006
Last update date: Jul 6 2016
Access: Public
Diseases: Autonomic Nervous System Diseases
Dataset link LD/DD time course of y w; tim01 Drosophila #2

Experimental Protocol

y w; tim01 flies that had been kept in a 12-hr light/ 12-hr dark cycle for three days were harvested every four hours during an additional light/dark day (ZT) and a subsequent day in constant darkness (CT). Relative to Zeitgeber time 0 (ZT0) as the time of lights on during the LD cycle and Circadian time 0 (CT0) as the time corresponding to subjective lights-on during freerun in DD, time courses were collected in a ZT2- ZT6-ZT10-ZT14-ZT18-ZT22-CT2-CT6-CT10-CT14-CT18-CT22 schedule. Heads were isolated by breaking up frozen flies and passing them through a set of sieves. RNA was prepared using guanidine-thiocyanate extraction followed by purification over a CsCl gradient. Additional purification of the RNA samples was achieved by applying them to Rneasy columns (Qiagen). Biotin-labeled cRNA probe was generated from 25 μg of purified RNA and hybridized as described previously (Wijnen H, Naef F, and Young MW, Methods Enzymol. 2005; 393: 341-365). For more information see also










Herman Wijnen