Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Oct 1 2018
Last update date: Oct 29 2018
Access: Public
Genes: CWC27
Taxon: Homo sapiens
Dataset link Comparison of CWC27 knockdown in HEK293T cells compared to scrambled shRNA control

Experimental Protocol


3 replicates of CWC27 knockdown cell lines (HEK293T cells, stably integrated, selected using puro, MISSION shRNA TRCN0000000139: 5'-CCGGACGGGAACTCTTAGCAGCAAACTCGAGTTTGCTGCTAAGAGTTCCCGTTTTTT-3'). 3 replicates of SCR control cell lines (HEK293T cells, stably integrated, selected using puro, sequence 5′-CCTAAGGTTAAGTCGCCCTCGCTCTAGCGAGGGCGACTTAACCTT-3′). total RNA purified from ~1 million cells, cDNA converted using random hexamer primers, quantity measured using NanoDrop, quality quantified using BioAnaylzer. Affymatrix HTA2.0 array

Repositories


GEO

GSE117144

BioProject

PRJNA481269

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Contact


Tara Davis