|Application:||Gene expression microarray analysis|
|Number of samples:||15|
|Release date:||Oct 31 2012|
|Last update date:||Dec 6 2012|
|Diseases:||Dog Diseases, Muscular Diseases, Muscular Dystrophies, Muscular Dystrophy, Duchenne|
|Dataset link||Gene expression profiles using microarrays in diaphragm of normal Beagle dogs and Beagle-based canine muscular dystrophy (CXMDJ) before and 1 hour after initial respiration|
Diaphragm were isolated from four groups (normal Beagle dogs and CXMDJ before and 1 hour after initial respiration). Total RNA was purified and prepared to Agilent-021193 Canine (V2) Gene Expression Microarray (Feature Number version) (Agilent Technologies) using Agilkent reagents and protocols. The mRNA levels of differential expressed genes from gene chip analysis were confirmed by quantitative real-time PCR assay.