Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Sep 13 2012
Last update date: Sep 14 2012
Access: Public
Dataset link Transcriptome analysis of the WalKR regulon in Staphylococcus aureus

Experimental Protocol


Cells were grown in TSB rich medium added with 0.25 µM CdCl2 to allow walRc expression until OD600nm of 1. Gene expression was compared between the HG001 wild type reference strain carrying the pCN51 empty vector (HG001/pCN51) and the HG001 strain carrying a pCN51 derivative with the walRc allele under the control of the Pcad inducible promoter (HG001/pSD3-12). Each condition was done in triplicate and the data were analyzed using the Arraystat software. Genes were considered as differentially expressed if their transcriptional level differed by at least two-fold with a Z-test P-value ≤ 0.05 in at least two of the replicates.

Repositories


GEO

GSE29337

ArrayExpress

E-GEOD-29337

BioProject

PRJNA140141

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Contact


Ulrike Mäder
Ulrike Mäder