|Application:||RNA-seq analysis, BS-seq analysis|
|Number of samples:||100|
|Release date:||Mar 15 2018|
|Last update date:||Nov 28 2018|
|Dataset link||Dissecting the Functional Consequences of de Novo DNA Methylation Dynamics in Human Motor Neuron Differentiation and Physiology|
We performed whole genome bisulfite sequencing and RNA-Seq on 3 time points during in vitro differentiation of human embryonic stem cells towards spinal motor neurons at day 0, day 6 and day 14 in three conditions, WT, DNMT3A and DNMT3B knockout backround in 3-5 replicates each. In addition, we also performed single cell RNA-Seq on the same conditions and time point 6 and 14 as well as bisulfite amplicon sequencing. Please note that raw data is to be made available through dbGaP (controlled access) due to privacy concerns.