Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Jul 21 2009
Last update date: Mar 21 2012
Access: Public
Dataset link Gene expression and synergistic interactions induced by CpG ODN and/or poly(I:C)

Experimental Protocol


RAW 264.7 cells were cultured for 5 days in DMEM supplemented with 10% FCS, 50 U/ml penicillin, 50 ug/ml streptomycin, and 1mM sodium pyruvate at 37C in a 5% CO2 in air incubator. Cells were washed, transferred into new flasks, and rested for 16 hr before being stimulated with 3.2 ug/ml CpG ODN and/or 32 ug/ml poly(I:C) for 4 - 12 hr. All experiments were independently repeated three times, and data from all experiments combined for analysis. Control samples included RAW 264.7 cells that were unstimulated and collected at 4 and 12 hours. All arrays used reverse transcribed mRNA from stimulted and unstimulated RAW cells as well as Universal Mouse Reference RNA purchased from Stratagene, La Holla, CA.

Repositories


GEO

GSE15416

ArrayExpress

E-GEOD-15416

BioProject

PRJNA115821

Download


Contact


Debra Tross