Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 17
Release date: Apr 11 2013
Last update date: Jul 19 2017
Access: Public
Diseases: Hematologic Neoplasms
Genes: ITGA2B, GATA1, ADH, TGFB1
Dataset link Gene expression analyses of hematopoietic stem cell (HSC) subsets in wildtype or CD41-KO mice

Experimental Protocol


For microarray analysis, 1000 HSCs were FACS sorted from pools of 4-5 mice directly into lysis buffer, RNA extracted and whole transcript amplification was performed as previously described (Gonzalez-Roca E, Garcia-Albeniz X, Rodriguez-Mulero S, Gomis RR, Kornacker K, Auer H. Accurate expression profiling of very small cell populations. PLoS One. 2010;5:e14418.)

Repositories


GEO

GSE45561

ArrayExpress

E-GEOD-45561

BioProject

PRJNA194578

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Contact


Christos Gekas