Computational protocol: Differential Host Immune Responses after Infection with Wild-Type or Lab-Attenuated Rabies Viruses in Dogs

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Protocol publication

[…] The blood and lymph node aspirates were collected before and after infection. Briefly, PBMCs were isolated from 10ml of blood using Ficoll Histopaque solution (Sigma-Aldrich). Then, cells were stained with CD3, CD4, CD8, CD19, CD40, CD11c and CD86 antibodies and isotype control. Data collection and analysis were performed using a BD LSR-II flow cytometer, BD FACSDiva software (BD Pharmingen), and FlowJo software (TreeStar, San Carlos, CA). […]

Pipeline specifications

Software tools BD FACSDiva, FlowJo
Application Flow cytometry
Organisms Canis lupus familiaris, Viruses, Human poliovirus 1 Mahoney, Dolphin rhabdovirus