Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 17
Release date: May 17 2015
Last update date: May 22 2015
Access: Public
Diseases: Melanoma, Neoplasms
Dataset link A microfluidic device for label-free, physical capture of circulating tumor cell-clusters

Experimental Protocol


We used the Cluster-Chip to capture CTC-clusters from the blood of a breast cancer patient with high CTC counts, released CTC-clusters in solution, stained them with TexasRed-conjugated antibodies against the leukocyte cell surface markers CD45, CD14 and CD16, and then isolated intact CTC-clusters individually using a micromanipulator. From a single time point, we retrieved 15 CTC-clusters, and each of those clusters was individually subjected to RNA-sequencing analysis using a next generation platform (SOLiD 5500). In addition, two leukocytes were isolated from the blood of a healthy donor were individually subjected to RNA-sequencing analysis using the same platform.

Repositories


GEO

GSE67939

ArrayExpress

E-GEOD-67939

ENA

SRP057212

BioProject

PRJNA281253

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Contact


Ben Wittner
Ben S. Wittner

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