Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 18
Release date: Aug 12 2016
Last update date: Sep 21 2018
Access: Public
Genes: FGF9, IL6
Dataset link Transcription factors GAF and HSF act at distinct regulatory steps to modulate stress-induced gene activation

Experimental Protocol


We used PRO-seq to map the distribution of transcriptionally engaged RNA polymerases in Drosophila S2 cells before (non-Heat Shock) and after 20min of Heat Shock in LacZ-RNAi (control), GAF-RNAi, HSF-RNAi and M1BP-RNAi treated cells. We also performed PRO-seq as a function of time after Heat Shock induction (30sec, 2min, 5min, 10min and 20min) in S2 cells. Furthermore, we used RNA-seq to measure mRNA levels in S2 cells before (non-Heat Shock) and after 30min of Heat Shock. All experiments were performed in two biological replicates.

Repositories


GEO

GSE77607

ENA

SRP069335

BioProject

PRJNA311005

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Contact


Fabiana Duarte