|Number of samples:||18|
|Release date:||Aug 12 2016|
|Last update date:||Sep 21 2018|
|Dataset link||Transcription factors GAF and HSF act at distinct regulatory steps to modulate stress-induced gene activation|
We used PRO-seq to map the distribution of transcriptionally engaged RNA polymerases in Drosophila S2 cells before (non-Heat Shock) and after 20min of Heat Shock in LacZ-RNAi (control), GAF-RNAi, HSF-RNAi and M1BP-RNAi treated cells. We also performed PRO-seq as a function of time after Heat Shock induction (30sec, 2min, 5min, 10min and 20min) in S2 cells. Furthermore, we used RNA-seq to measure mRNA levels in S2 cells before (non-Heat Shock) and after 30min of Heat Shock. All experiments were performed in two biological replicates.