Computational protocol: Increased Expression of SETD7 Promotes Cell Proliferation by Regulating Cell Cycle and Indicates Poor Prognosis in Hepatocellular Carcinoma

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Protocol publication

[…] After SMMC-7721 cells was transfected with si-SETD7-2 or the scrambled RNA for 24 h, the cells were harvested in TRIzol reagent (SETD7 knockdown efficiency were confirmed) then sent to DGE analysis. Sequencing was performed at RiboBio Co., Guangzhou through the illumineHiSeq2500. Data was aligned to the Ensembl transcript annotations using bowtie and RSEM as described previously []. All other bioinformatics analysis was performed using glbase []. […]

Pipeline specifications

Software tools Bowtie, RSEM, Glbase
Application DGE analysis
Organisms Homo sapiens
Diseases Carcinoma, Hepatocellular, Liver Neoplasms, Neoplasms, Soft Tissue Injuries