Computational protocol: Effects of N-Cadherin Disruption on Spine Morphological Dynamics

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[…] Raw image stacks were 3D deconvolved in IMAGEJ [NIH, “Iterative Deconvolve 3D” plugin (Dougherty, )] using a theoretical estimate of the 3-D PSF (IMAGEJ, “Diffraction PSF 3D” plugin). No further filtering was performed. Stacks at all time-points were registered to the first stack. The z-projection (maximum) of the stack at each time-point was obtained, and from this, dendrites were straightened in IMAGEJ. The images at this stage are referred to as raw images in the text. […]

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