Computational protocol: Selective inhibition of HDAC8 decreases neuroblastoma growth in vitro and in vivo and enhances retinoic acid-mediated differentiation

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Protocol publication

[…] In six-well plates, 500 cells were seeded and treated as indicated. Viable colonies were stained after a minimum of 10 days with crystal violet. For quantification, the mean intensity of each well of the 8-bit binary picture was measured with ImageJ software (U. S. National Institutes of Health, Bethesda, MD, USA; http://imagej.nih.gov/ij/). [...] For in vitro experiments, either a two-sided t-test or an one-way analysis of variance was carried out (SigmaPlot 12.0). Means are represented in bar charts, and error bars represent the S.D. of at least three independent experiments. P-values <0.05 were considered significant. For assessment of in vivo effects, tumor volumes were log-transformed. A linear mixed model was used with fixed slope for each group and random intercept for each mouse. Statistical analysis of additive or synergistic effects was performed using SAS Proc Mixed (Version 9.2; SAS Institute, Cary, NC, USA). Tumor weights were analyzed with the nonparametric Mann–Whitney test (GraphPad Prism version 3.0a). Error bars represent the S.E.M. P-values <0.05 were considered significant. […]

Pipeline specifications

Software tools ImageJ, SigmaPlot
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Mus musculus
Diseases Neoplasms, Neuroblastoma, Drug-Related Side Effects and Adverse Reactions
Chemicals Tretinoin