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Specifications


Application: Gene expression microarray analysis
Number of samples: 20
Release date: Feb 20 2008
Last update date: Mar 16 2012
Access: Public
Chemicals: Ethanol, Phenol
Dataset link Acid-Shifted Time Course of Gene Expression Profiles of Aerobically Grown Escherichia coli K-12

Experimental Protocol


Gene expression profiles of Escherichia coli K-12 W3110 were compared as a function of time versus change in external pH. Overnight cultures were diluted 1:500 in potassium-modified Luria-Bertani medium (LBK) buffered with 20 mM HOMOPIPES at 7.6. Bacteria were cultured in baffled flasks (less than 10% volume) with rotation at 225 rpm, incubated at 37°C to an optical density at 600 nm of 0.2. External pH was lowered to pH 5.5 by adding 840 µl of 1M HCl to 40 mls of culture within 5 seconds. Four samples were taken from each of five replicate cultures, corresponding to time 0 (before HCl addition),1, 5, and 10 min post HCl addition.

Repositories


GEO

GSE4778

ArrayExpress

E-GEOD-4778

BioProject

PRJNA95551

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Contact


Joan Slonczewski
Joan Lyn Slonczewski

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