|Application:||Gene expression microarray analysis|
|Number of samples:||20|
|Release date:||Feb 20 2008|
|Last update date:||Mar 16 2012|
|Dataset link||Acid-Shifted Time Course of Gene Expression Profiles of Aerobically Grown Escherichia coli K-12|
Gene expression profiles of Escherichia coli K-12 W3110 were compared as a function of time versus change in external pH. Overnight cultures were diluted 1:500 in potassium-modified Luria-Bertani medium (LBK) buffered with 20 mM HOMOPIPES at 7.6. Bacteria were cultured in baffled flasks (less than 10% volume) with rotation at 225 rpm, incubated at 37°C to an optical density at 600 nm of 0.2. External pH was lowered to pH 5.5 by adding 840 µl of 1M HCl to 40 mls of culture within 5 seconds. Four samples were taken from each of five replicate cultures, corresponding to time 0 (before HCl addition),1, 5, and 10 min post HCl addition.
Joan Lyn Slonczewski
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