|Application:||Gene expression microarray analysis|
|Number of samples:||3|
|Release date:||Apr 4 2016|
|Last update date:||Apr 15 2016|
|Chemicals:||Iron, Glutamic Acid, Succinic Acid|
|Dataset link||C.glutamicum Δpup mutant senses a stronger iron limitation than the wild type.|
DNA microarray analyses were performed to compare the mRNA levels of the C. glutamicum Δpup mutant and its parent wild type under iron-limited conditions. The two strains precultivated in CGXII medium with 4% (w/v) glucose and 1 µM FeSO4 were inoculated into fresh medium to an OD600 of 1, cultured for 2 h, and harvested on ice by centrifugation (5 min at 4,000 g and 4°C). Please note that the GPL16989 array design comprises oligos for 4 different bacterial genomes. In the GPR-files in this study, all IDs/Names (oligonucleotides) which are not from the host C. glutamicum were replaced by the text EMPTY since only C. glutamicum expression was analyzed.