Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 3
Release date: Apr 4 2016
Last update date: Apr 15 2016
Access: Public
Chemicals: Iron, Glutamic Acid, Succinic Acid
Dataset link C.glutamicum Δpup mutant senses a stronger iron limitation than the wild type.

Experimental Protocol


DNA microarray analyses were performed to compare the mRNA levels of the C. glutamicum Δpup mutant and its parent wild type under iron-limited conditions. The two strains precultivated in CGXII medium with 4% (w/v) glucose and 1 µM FeSO4 were inoculated into fresh medium to an OD600 of 1, cultured for 2 h, and harvested on ice by centrifugation (5 min at 4,000 g and 4°C). Please note that the GPL16989 array design comprises oligos for 4 different bacterial genomes. In the GPR-files in this study, all IDs/Names (oligonucleotides) which are not from the host C. glutamicum were replaced by the text EMPTY since only C. glutamicum expression was analyzed.

Repositories


GEO

GSE64866

ArrayExpress

E-GEOD-64866

BioProject

PRJNA272420

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Contact


Tino Polen