Computational protocol: Methionine Biosynthesis is Essential for Infection in the Rice Blast Fungus Magnaporthe oryzae

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Protocol publication

[…] Available fungal genome sequences were searched using different Blast algorithms at the NCBI (, Broad Institute ( and JGI ( websites. The M. oryzae genome sequence version 8 and the corresponding CDS and gene nomenclature (v7) available at the Broad Institute, were used in this study. M. oryzae ESTs were searched using BlastN at the NCBI and COGEME ( websites. Protein sequence alignments and phylogenetic trees were obtained using ClustalW and MEGA 5.1 []. […]

Pipeline specifications

Software tools BLASTN, Clustal W, MEGA
Databases COGEME
Application Phylogenetics
Organisms Magnaporthe oryzae, Hordeum vulgare, Oryza sativa
Diseases Infection
Chemicals Cystathionine, Cysteine, Glutathione, Homocysteine, Methionine, S-Adenosylhomocysteine, S-Adenosylmethionine, Vitamin U