Computational protocol: The novel component Kgd4 recruits the E3 subunit to the mitochondrial α-ketoglutarate dehydrogenase

Similar protocols

Protocol publication

[…] For visualization purposes, we constructed a phylogenetic tree of Kgd4 homologues. A multiple sequence alignment was generated using MUSCLE () and the regions of poor alignment were removed using Gblocks (; maximum nonconserved positions = 8; minimum block length = 5). The tree was constructed using PhyML 3.0 (; Jones–Taylor–Thornton matrix) with 100 bootstrap replicates and visualized using iTOL (http://itol.embl.de). […]

Pipeline specifications

Software tools MUSCLE, Gblocks, PhyML, iTOL
Applications Phylogenetics, Nucleotide sequence alignment
Chemicals Citric Acid