Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Feb 23 2011
Last update date: Mar 22 2012
Access: Public
Chemicals: Estradiol, Estrogens
Genes: MYB, CAGE1, ERAL1
Dataset link Defining the Myb Transcriptional Program Controlled by via Genome-wide Chromatin Occupancy and Expression Analyses

Experimental Protocol


Triplicate total RNA samples from ERMYB cells were collected at 0, 6, and 24 hr after β-E2 withdrawal and 6 hr after β-E2 re-addition. Samples were analyzed by Illumina Mouse Whole Genome arrays (WG6). Probe intensities were variance stabilised and normalized by the Lumi package (Du et al., 2008). Differentially expressed genes were identified by the LIMMA package (Smyth, 2004) and then grouped into different classes according to their kinetic profiles.

Repositories


GEO

GSE22486

ArrayExpress

E-GEOD-22486

BioProject

PRJNA128699

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Contact


paul LEO
paul james LEO

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