|Application:||RNA-seq analysis, sRNA-seq analysis|
|Number of samples:||3|
|Release date:||May 10 2011|
|Last update date:||Jun 11 2013|
|Taxon:||Panicum virgatum, Zea mays|
|Dataset link||High-throughput sequencing of small RNAs in Panicum virgatum|
Small RNA libraries were derived from leaves, drought-treated leaves and flowers of Panicum virgatum. Total RNA was isolated using the Plant RNA Purification Reagent (Invitrogen), and submitted to Illumina (Hayward, CA, http://www.illumina.com) for small RNA library construction using approaches described in (Lu et al., 2007) with minor modifications. The small RNA libraries were sequenced with the Sequencing-By-Synthesis (SBS) technology by Illumina. PERL scripts were designed to remove the adapter sequences and determine the abundance of each distinct small RNA. We thank Pamela Green for providing the plant material as well as Kan Nobuta and Gayathri Mahalingam for assistance with the computational methods.
Emanuele De Paoli