Computational protocol: Peptide-Major Histocompatibility Complex Dimensions Control Proximal Kinase-Phosphatase Balance during T Cell Activation

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Protocol publication

[…] The Metamorph and ImageJ software packages were used for all image processing, and ImageJ was used for calculation of the Manders coefficient. All of the images were background subtracted prior to analysis. Interface enrichment was calculated as previously described using Metamorph (). […]

Pipeline specifications

Software tools MetaMorph, ImageJ
Application Microscopic phenotype analysis
Organisms Mus musculus, Homo sapiens
Chemicals Receptor-CD3 Complex, Antigen, T-Cell