|Application:||RNA-seq analysis, sRNA-seq analysis, ChIP-seq analysis|
|Number of samples:||17|
|Release date:||Oct 15 2011|
|Last update date:||Sep 13 2013|
|Dataset link||Glucosylated hydroxymethyluracil (DNA base J) prevents transcriptional read-through in Leishmania|
We determined the exact location of base J in the genome of Leishmania by high-throughput sequencing of J containing DNA fragments. Samples were enriched for J-containing fragments by two independent methods: ChIP using an anti-J DNA antibody or by binding to a the J-binding protein JBP1. We studied the effect of loss of J on transcription using WT, JBP2 knockout (30-37% of WT level J), and JBP2 knockout cells grown in BrdU containing medium (13-16% of WT level of J). We used 6 RNA-seq libraries (three samples & two replica each) containing processed RNA products (transspliced and poly-adenylated) and 3 small RNA libraries representing the entire transcriptome.