Computational protocol: The membrane tethered transcription factor EcbZIP17 from finger millet promotes plant growth and enhances tolerance to abiotic stresses

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Protocol publication

[…] For nucleotide and protein sequence analysis, the National Centre for Biotechnology Information (NCBI; was used. The basic leucine zipper (BRLZ) and transmembrane domains in EcbZIP17 were observed using the Simple Modular Architecture Research Tool (SMART; The leucine rich nuclear export signals (NES) were predicted by the NetNES 1.1 server (, whereas the nuclear localisation signal was predicted by the cNLS Mapper tool ( The CELLO 2.5 Sub-Cellular Localisation predictor ( was used for subcellular localisation. The alignment analysis of EcbZIP17 and its homologs obtained from related species was carried out using CLUSTALW2 (, and the phylogenetic tree was constructed using MEGA 5.0. The Multiple EM for Motif Elicitation software (MEME; was used for identifying motifs present in CLUSTALW2 aligned sequences. […]

Pipeline specifications

Software tools NetNES, cNLS Mapper, CELLO, Clustal W, MEME, MEME Suite
Databases SMART
Applications Phylogenetics, Protein sequence analysis
Organisms Nicotiana tabacum
Diseases Machado-Joseph Disease
Chemicals Mannitol, Polyethylene Glycols, Sodium Chloride