Computational protocol: Beneficial Effects of Evogliptin, a Novel Dipeptidyl Peptidase 4 Inhibitor, on Adiposity with Increased Ppargc1a in White Adipose Tissue in Obese Mice

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Protocol publication

[…] Epididymal adipose tissues were removed, prepared for 4 μm-thick microtome sections, and then mounted for hematoxylin-eosin staining. Images were obtained using an Axioskop2 Plus microscope (Carl Zeiss, Gottingen, Germany), and adipocyte area was measured using ImageJ (http://rsbweb.nih.gov/ij). After processing background of images, the cellular boundary line was automatically detected and manually compared to the original image. Then the area of each cell in the same area was automatically determined except for cells appearing partly at the edge of image frame. Three sections per animal were analyzed. [...] All data was expressed as means ± SE. Except for ANCOVA, statistical analyses were performed using SigmaStat® 2.0 (SPSS, Chicago, IL). Comparisons with baseline values and comparisons between two groups were analyzed using Student’s paired t-test and Student’s t-test, respectively. For comparisons of data involving more than three groups at the same time point, one-way analysis of variance (ANOVA) was used. For comparisons of data at different time points from the same animals among more than three groups, we used repeated measures (RM) two-way ANOVA. When ANOVA indicated a significant difference, the differences were evaluated further using Dunnett’s multiple comparison test. The adipocyte size distribution was analyzed using the Mann—Whitney rank sum test between two groups due to data skewness. P values < 0.05 were considered significant. One-way analysis of covariance (ANCOVA) was performed using SigmaPlot® 13.0 (Systat Software, Inc., San José, CA). […]

Pipeline specifications

Software tools ImageJ, SPSS, SigmaPlot
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Mus musculus
Diseases Hypotension, Neoplasms, Adipose Tissue