|Number of samples:||6|
|Release date:||May 16 2010|
|Last update date:||Mar 21 2012|
|Taxon:||Homo sapiens, Mus musculus|
|Dataset link||Repressive and active histone methylation mark distinct promoters in human and mouse spermatozoa (Nimblegen)|
Chromatin immuno precipitation (ChIP) was performed on sperm samples obtained from 9 normospermic donors. DNA associated with H3K4me2 or H3K27me3 was precipitated using specific antibodies. Input and precipitated DNA were amplified and hybridized to a tiling microarray (NimbleGen Systems Inc.) representing 18029 promoter regions (2200bp upstream to 500bp downstream of transcription start sites) of all RefSeq annotated human genes. For each modification 3 independent ChIP experiments were performed of which one was hybridized in a dye swap configuration.