Dataset features

Specifications


Application: ChIP-on-chip analysis
Number of samples: 6
Release date: May 16 2010
Last update date: Mar 21 2012
Access: Public
Taxon: Homo sapiens, Mus musculus
Dataset link Repressive and active histone methylation mark distinct promoters in human and mouse spermatozoa (Nimblegen)

Experimental Protocol


Chromatin immuno precipitation (ChIP) was performed on sperm samples obtained from 9 normospermic donors. DNA associated with H3K4me2 or H3K27me3 was precipitated using specific antibodies. Input and precipitated DNA were amplified and hybridized to a tiling microarray (NimbleGen Systems Inc.) representing 18029 promoter regions (2200bp upstream to 500bp downstream of transcription start sites) of all RefSeq annotated human genes. For each modification 3 independent ChIP experiments were performed of which one was hybridized in a dye swap configuration.

Repositories


GEO

GSE13863

ArrayExpress

E-GEOD-13863

BioProject

PRJNA123771

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Contact


Antoine Peters