|Application:||Gene expression microarray analysis|
|Number of samples:||8|
|Release date:||Feb 20 2008|
|Last update date:||Mar 17 2012|
|Computational protocol:||SAM, Primer3, GEO|
|Dataset link||Discovery of genes associated with whirling disease infection and resistance in rainbow trout using expression profiling|
Resistant (Hofer) and susceptible (Trout Lodge) rainbow trout strains were exposed to 2,000 triactinomyxons (whirling disease pathogen) per fish. Unexposed controls for each strain were treated identically to exposed other than their lack of pathogen exposure. After twenty-four hours, caudal fin tissue was collected for RNA extraction. Amplified RNA was competitively hybridized (exposed versus control) for each strain. Four biological replicates were used for each experimental condition and a balanced block design was incorporated.
DOI: 10.1186/1471-2164-9-37call_split See protocol