Dataset features

Specifications


Dataset type: Expression profiling by RT-PCR, Transcriptome or Gene expression
Number of samples: 9
Release date: Nov 10 2016
Last update date: Nov 10 2016
Access: Public
Diseases: Celiac Disease
Dataset link Dietary Gluten-Induced Gut Dysbiosis is Accompanied by Selective Upregulation of microRNAs with Intestinal Tight Junction and Bacteria-Binding Motifs in Rhesus Macaque Model of Celiac Disease

Experimental Protocol


Four age and weight matched gluten sensitive (GS) Indian rhesus macaques were fed gluten containing monkey chow. Five rhesus macaques served as normal healthy controls. Jejunal segments were collected at necropsy from both groups. All animals were necropsied at 60 days post SIV infection. ~100 ng of total RNA was first reverse transcribed and preamplified according to the manufacturer’s recommendation. microRNA expression profiling was performed using TaqMan ®OpenArray® Human microRNA panels. Data analysis was performed using ExpressionSuite® software. Data was normalized to two endogenous controls (RNU44 and RNU48). Delta CT values were calculated by subtracting individual miRNA CT values from an average of both endogenous controls. Comparisons were made between gluten sensitive and normal healthy control macaques. Four age and weight matched gluten sensitive Indian rhesus macaques were fed gluten containing monkey chow. Five rhesus macaques served as normal healthy controls. Jejunal segments were collected at necropsy from both groups. All animals were necropsied at 60 days post SIV infection. ~100 ng of total RNA was first reverse transcribed and preamplified according to the manufacturer’s recommendation. microRNA expression profiling was performed using TaqMan ®OpenArray® Human microRNA panels. Data analysis was performed using ExpressionSuite® software. Data was normalized to two endogenous controls (RNU44 and RNU48). Delta CT values were calculated by subtracting individual miRNA CT values from an average of both endogenous controls. Comparisons were made between gluten sensitive and normal healthy control macaques. Four age and weight matched gluten sensitive Indian rhesus macaques were fed gluten containing monkey chow. Five rhesus macaques served as normal healthy controls. Jejunal segments were collected at necropsy from both groups. All animals were necropsied at 60 days post SIV infection. ~100 ng of total RNA was first reverse transcribed and preamplified according to the manufacturer’s recommendation. microRNA expression profiling was performed using TaqMan ®OpenArray® Human microRNA panels. Data analysis was performed using ExpressionSuite® software. Data was normalized to two endogenous controls (RNU44 and RNU48). Delta CT values were calculated by subtracting individual miRNA CT values from an average of both endogenous controls. Comparisons were made between gluten sensitive and normal healthy control macaques.

Repositories


GEO

GSE89170

BioProject

PRJNA350519

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Contact


Mahesh Mohan