Computational protocol: Amyloid domains in the cell nucleus controlled by nucleoskeletal protein lamin B1 reveal a new pathway of mercury neurotoxicity

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Protocol publication

[…] The PANTHER (Protein ANalysis THrough Evolutionary Relationships) database v8.0 () was used to identify the cellular and molecular functions of the SDS-insoluble aggregate components. The annotation PANTHER protein class was used as a basic classification, and if necessary, revised by a manual modification of class names and protein class affiliation, based on literature and UniProtKB references. [...] The number of known interaction partners of each protein was extracted from the HIPPIE (Human Integrated Protein–Protein Interaction rEference) database v1.5 (). The database was imported to Microsoft Excel and the overall numbers of known interactions of each protein were calculated. The Mann–Whitney test was used to test for significant differences between the groups using OriginPro 8.6 (Originlab). […]

Pipeline specifications

Software tools PANTHER, HIPPIE
Applications Protein sequence analysis, Protein interaction analysis
Diseases Blood Protein Disorders, Neurotoxicity Syndromes
Chemicals Mercury