Dataset features

Specifications


Application: ChIP-seq analysis, WGS analysis
Number of samples: 71
Release date: Mar 20 2017
Last update date: Oct 24 2018
Access: Public
Diseases: Leukemia, Neoplasms, Disorders of Sex Development, Leukemia, Myeloid, Acute
Dataset link Genome-wide mapping of histone H3 lysine 9 dimethylation in normal myeloid cells and acute myeloid leukemia

Experimental Protocol


ChIP-Seq with antibodies against H3K9me2 was used with normal human granulocytes (2 samples), CD34+ hematopoietic progenitors (2 samples), K562 cells (2 intact samples and 2 samples treated with G9a inhibitor UNC0638), and primary myeloblasts from 8 different acute myeloid leukemia samples. Each H3K9me2 ChIP-seq was repeated twice. In addition, control experiments for antibody specificity were conducted with ChIP input DNA (16 samples), and ChIP-seq with antibodies against histone H3K9me3 (4 samples), histone H3K4me2 (1 sample), and nonmodified histone H3 C-tail (2 samples).

Repositories


GEO

GSE71809

ENA

SRP062133

BioProject

PRJNA292119

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