Computational protocol: Global analysis of genome, transcriptome and proteome reveals the response to aneuploidy in human cells

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Protocol publication

[…] Freshly cultured cell were seeded sparsely in a 6 channel μ-slide (ibidi, Martinsried, Germany) 24 h before the experiment. Time laps movies were taken by imaging asynchronous cells in a 10 min or 4 min interval for 72 or 48 h, respectively. The slide was placed onto a sample stage with an incubator chamber (EMBLEM, Heidelberg, Germany) maintained at a 37°C, 40% humidity, in an atmosphere of 5% CO2. Imaging was performed using a Zeiss Axio Observer Z1 microscope equipped with a Plan Neofluar 20 × air objective. Metamorph 7.1 software (Molecular Devices) was used to control the microscope. Movies were evaluated using the ImageJ 1.42I software. […]

Pipeline specifications

Software tools MetaMorph, ImageJ
Applications SPIM, Microscopic phenotype analysis
Organisms Homo sapiens