Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 4
Release date: Feb 4 2013
Last update date: Nov 3 2017
Access: Public
Taxon: Caenorhabditis elegans
Dataset link Identification of Transcription Factor NHR-77::GFP Binding Regions in L1

Experimental Protocol


EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: OP353(official name : OP353 genotype : unc119(ed3);wgIs353(nhr-77::TY1 EGFP FLAG;unc119) outcross : 3 transgene : nhr-77 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The NHR-77::EGFP fusion protein is expressed in the correct nhr-77 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-77 transcription factor. made_by : R Waterston ); Developmental Stage: fed L1; Genotype: unc119(ed3);wgIs353(nhr-77::TY1 EGFP FLAG;unc119); Sex: Hermaphrodite; Transgene: nhr-77; EXPERIMENTAL FACTORS: Developmental Stage fed L1; Target gene nhr-77; Strain OP353(official name : OP353 genotype : unc119(ed3);wgIs353(nhr-77::TY1 EGFP FLAG;unc119) outcross : 3 transgene : nhr-77 tags : Bombard tag : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The NHR-77::EGFP fusion protein is expressed in the correct nhr-77 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-77 transcription factor. made_by : R Waterston ); temp (temperature) 20 degree celsius

Repositories


GEO

GSE44012

ArrayExpress

E-GEOD-44012

ENA

SRP018544

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DCC modENCODE