Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 17
Release date: Sep 25 2017
Last update date: Oct 24 2018
Access: Public
Diseases: Encephalomyelitis, Autoimmune, Experimental
Dataset link Novel neuroprotective and neurogenic phenotype of microglia

Experimental Protocol


The overall design was to compare transcriptomes of subsets of microglia isolated from neonatal mice, healthy adults, and adult mice with a neuroinflammatory disease (Experimental autoimmune encephalomyelitis, EAE), and to compare anti-inflammatory function of adult and neonatal microglia. Microglia were isolated by cell-sorting based on surface phenotype, and RNAseq data was analyzed using WGCNA, GO and DAVID approaches. Expression of selected genes and pathways was confirmed by histology and flow cytometry. Functional analysis involved transfer of isolated microglia to the central nervous system of animals with EAE and evaluation of outcome. EAE = Experimental autoimmune encephalomyelitis

Repositories


GEO

GSE78809

ENA

SRP071039

BioProject

PRJNA313971

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Contact


Inge Holtman

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