Dataset features

Specifications


Dataset type: Other
Number of samples: 24
Release date: Feb 6 2016
Last update date: Feb 7 2016
Access: Public
Diseases: Neoplasms, Prostatic Neoplasms
Genes: CBX8, PROM1, PIK3CD, AKT1
Dataset link Expression profiling of 22Rv1, DU145, LNCaP and PC3 prostasphere cultures at day 0, 4, 8 and 12 [Cancer Reference]

Experimental Protocol


22Rv1, DU145, LNCaP and PC3 prostasphere cultures were sampled on Day 4 (P 2), Day 8 (P 3) and Day 12 (P 4), and compared relative to parental monolayers on Day 0 of culture (P 1). Three independent measurements were carried out for monolayers at Day 0 using the CancerReference Kit. Day 12 prostaspheres were enzymatically dissociated and sorted via MACS using the Anti-CD133/2 (293C3)-PE Antibody (Miltenyi) into CD133+ and CD133- fractions. Total RNA was extracted from CD133+ cells and analyzed using the nCounter GX Cancer Reference kit, the Stem Cell panel and the custom made ITESM CodeSet from NanoString Technologies. This series contains the Cancer Reference kit data.

Repositories


GEO

GSE77612

ArrayExpress

E-GEOD-77612

BioProject

PRJNA311031

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Contact


Roberto Portillo Lara