|Number of samples:||24|
|Release date:||Feb 6 2016|
|Last update date:||Feb 7 2016|
|Diseases:||Neoplasms, Prostatic Neoplasms|
|Genes:||CBX8, PROM1, PIK3CD, AKT1|
|Dataset link||Expression profiling of 22Rv1, DU145, LNCaP and PC3 prostasphere cultures at day 0, 4, 8 and 12 [Cancer Reference]|
22Rv1, DU145, LNCaP and PC3 prostasphere cultures were sampled on Day 4 (P 2), Day 8 (P 3) and Day 12 (P 4), and compared relative to parental monolayers on Day 0 of culture (P 1). Three independent measurements were carried out for monolayers at Day 0 using the CancerReference Kit. Day 12 prostaspheres were enzymatically dissociated and sorted via MACS using the Anti-CD133/2 (293C3)-PE Antibody (Miltenyi) into CD133+ and CD133- fractions. Total RNA was extracted from CD133+ cells and analyzed using the nCounter GX Cancer Reference kit, the Stem Cell panel and the custom made ITESM CodeSet from NanoString Technologies. This series contains the Cancer Reference kit data.
Roberto Portillo Lara