Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Jul 31 2011
Last update date: Mar 21 2012
Access: Public
Diseases: Chemical and Drug Induced Liver Injury
Chemicals: Potassium Dichromate, Hydroxyl Radical
Dataset link Transcriptomics evaluation of hexavalent chromium toxicity in human dermal fibroblasts

Experimental Protocol


16 samples were analyzed in this experiment. Exponentially growing human dermal fibroblasts (3x105 cells) were cultured in T25 cell culture flasks. When the cells were approximately 70% confluent, hexavalent potassium dichromate (5µM) was added to the medium with or without 40µM ferrous sulfate. Following 16 hours of culturing at 37oC, total RNA was isolated for gene expression studies. Details of the samples are: Control 4 Samples: Cr(0)-1, Cr(0)-2, Cr(0)-3, Cr(0)-4 Cr 5 µM 4 Samples: Cr(5)-5, Cr(5)-6, Cr(5)-7, Cr(5)-8 FeSO4 40 µM 4 Samples: Fe(40)-9, Fe(40)-10, Fe(40)-11, Fe(40)-12 Cr 5 µM + FeSO4 40 µM 4 Samples: Cr(5)+Fe(40)-13,Cr(5)+Fe(40)-14, Cr(5)+Fe(40)-15, Cr(5)+Fe(40)-16

Repositories


GEO

GSE16349

ArrayExpress

E-GEOD-16349

BioProject

PRJNA115303

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Contact


Rajendran Sellamuthu