Dataset features

Specifications


Application: BS-seq analysis
Number of samples: 2
Release date: Dec 22 2014
Last update date: Oct 10 2018
Access: Public
Diseases: Deficiency Diseases
Chemicals: 5-Methylcytosine
Computational protocol: Trim Galore!, Bismark, SeqMonk, DAVID, GEO, DDBJ
Dataset link Genome-wide bisulfite sequencing in zygotes identifies demethylation targets and maps the contribution of TET3 oxidation

Experimental Protocol


Three independent collections of zygotes were performed for each genotype (control and TET3 deletion), giving a total of 225 control and 237 TET3 deletion zygotes, of which 120 and 129 were derived from 129S2/SvHsd studs for control and TET3 samples, respectively. Zygotes were pooled and whole-genome bisulfite libraries were prepared using a post-bisulfite adaptor tagging strategy optimized from (Miura et al. Nucleic Acids Research 2012, 40:e136)

Repositories


GEO

GSE63417

ArrayExpress

E-GEOD-63417

ENA

SRP050005

BioProject

PRJNA267699

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Contact


Felix Krueger

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