Computational protocol: Non-immune immunoglobulins shield Schistosoma japonicum from host immunorecognition

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Protocol publication

[…] Only proteins detected more than two times within three biological replicates were included in the following analyses. Putative functions and sequence features were assigned using BLASTp against annotated proteins in the GenBank database, and the alignment was classified to be significant if the corresponding E values < 1E-5. The transmembrane proteins were predicted using TMHMM Server v.2.0 (http://www.cbs.dtu.dk/services/TMHMM/). InterPro domains were annotated by InterProScan (Release 27.0), and functional assignments were mapped onto Gene Ontology (GO). […]

Pipeline specifications

Software tools BLASTP, TMHMM, InterPro, InterProScan
Applications Protein sequence analysis, Membrane protein analysis
Organisms Schistosoma japonicum, Homo sapiens, Miscanthus sinensis
Diseases Multiple Sclerosis, Parasitic Diseases