Computational protocol: The Ribosomal Protein Rpl22 Controls Ribosome Composition by Directly Repressing Expression of Its Own Paralog, Rpl22l1

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Protocol publication

[…] Protein sequences for murine Rpl22 (NP_033105) and Rpl22l1 (NP_080793) were obtained from Genbank (http://www.ncbi.nlm.nih.gov/). Sequence alignments were performed using ClustalW2 Algorithm (http://www.ebi.ac.uk/Tools/msa/clustalw2/). mRNA sequences for mRpl22l1 (NM_026517) and zRpl22l1 (NM_001045335) were entered into mFold (http://mfold.rna.albany.edu/?q=mfold/RNA-Folding-Form) for prediction of RNA secondary structure. […]

Pipeline specifications

Software tools Clustal W, Mfold
Application RNA structure analysis
Organisms Mus musculus, Saccharomyces cerevisiae, Homo sapiens